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Genetic Variation of Black Capped White Eye Zosterops atricapilla (Aves: Zosteropidae) Based on Mitochondrial DNA Cytochrome b Gene
Deby Permana

Last modified: 2020-11-20

Abstract


The purpose of this research was to explore genetic variation of black capped white eye Zosterops articapilla using the mitochondrial DNA cytochrome b gene. Blood sample was collected via pectoral vein of bird from Panorama markets, Bengkulu city. DNA genome were isolated and purifiated from the blood following Dneasy protocol® Blood and Tissue Kit cat no. 69504 (50), based on Qiagen's Spin-Column Protocol procedure. We used polymerase chain reaction machine for amplification DNA template with specific primer (ZCYTBF and ZCYTBR). The results showed that length of cyt b gene sequence (n=6) was 725 bp and had 717 bp (98.89%) conservative site. The average of intraspecific genetic distance was 0.04, interspecific 0.063, and between Indonesian and overseas (outgroup) was 0.138.

Keywords


Cytochrome b, mtDNA, illegal trafficking, Zosteropidae